Biological source: human
Recombinant: expressed in E. coli
Mole Weight: ~ 34 kDa
Packaging: 500 μg pack
Storage condition: avoid repeated freeze/thaw cycles
Impurities: endotoxin, tested
NCBI access no: NM_000041
UniProt access no: P02649
Apolipoprotein E (ApoE) belongs to a group of proteins that reversibly bind to lipoproteins. Significant amounts of ApoE are produced in the liver and brain and, to some extent, in almost all organs. ApoE is an important component of all plasma lipoproteins. ApoE exists in three main isoforms; E2, E3, and E4, which differ from each other by a single amino acid substitution. Compared to E3 and E4, E2 exhibits the lowest receptor binding affinity.
Carriers of the E2 allele have significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol, as well as increased levels of ApoE. The gene encoding this protein is located on human chromosome 19q13.32. Recombinant human ApoE2 is a 34.3 kDa protein containing 300 amino acid residues.
Biochemical / Physiological Actions
In addition to facilitating lipid solubilization, apolipoproteins help maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Apolipoprotein E (ApoE) plays an important role in lipid metabolism. Its interaction with the specific ApoE receptor allows the uptake of chylomicron residues by liver cells, which is an essential step during normal lipid metabolism. It also binds to the LDL receptor (Apo B / E). ApoE defects are a cause of type III hyperlipoproteinemia.
Physical form: Sterile filtered and lyophilized without additives.
Preparation note: Centrifuge the vial before opening. Avoid freeze-thaw cycles.
Reconstitution: Reconstitute in dH2O at a concentration of 0.1-1.0 mg / ml. The solution can then be diluted in other aqueous buffers and stored at 4 ° C for 1 week or –20 ° C for future use.