Alternative name: Apolipoprotein E4, APOE, apolipoprotein, apolipoproteins
Source: E. coli
Appearance: Freeze-dried protein
Physical form Description: Sterile filtered and lyophilized without additives
Molecular weight: 34.4 kDa
Purity by SDS-PAGE: ≥90%
Purity at: ≥90%
Endotoxin Level: <0.1 ng / μg
Reconstitute in dH₂O at a concentration of 0.1-1.0 mg / ml. The solution can then be diluted in other aqueous buffers and stored at 4 ° C for 1 week or –20 ° C for future use.
Handling: Centrifuge the vial before opening.
Storage conditions: -20 ° C
Shipping conditions: Gel Pack
USE: For research use only! It should not be used in humans.
ApoE belongs to a group of proteins that reversibly bind to lipoproteins and play an important role in lipid metabolism. In addition to facilitating lipid solubilization, these proteins help maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism.
Significant amounts of ApoE are produced in the liver and brain and, to some extent, in almost all organs. ApoE is an important component of all plasma lipoproteins. Its interaction with the specific ApoE receptor allows the uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds to the LDL receptor (apo B / E). ApoE defects are a cause of type III hyperlipoproteinemia.
ApoE exists in three main isoforms; E2, E3, and E4, which differ from each other by a single amino acid substitution. Individuals heterozygous for the ApoE4 allele are at increased risk for late-onset Alzheimer’s disease. Recombinant human ApoE4 is a 34.4 kDa protein containing 300 amino acid residues (accession number AAB59397).