Ontwikkeling en toepassing van een nieuwe multiplex PCR-test voor de differentiatie van vier hemosporidische parasieten bij de kip Gallus gallus domesticus

Haemosporidische infecties bij gedomesticeerde kippen (Gallus gallus domesticus) komen niet alleen veel voor, maar veroorzaken ook economische schade. De diagnose wordt meestal gesteld door microscopisch onderzoek; de methode heeft echter verschillende nadelen, zoals het vereisen van een ervaren microscopist, onbetrouwbaar zijn wanneer parasitemie laag is en niet in staat zijn om nauwkeurig onderscheid te maken tussen co-infecties van meerdere parasietsoorten. Daarom kan de huidige omvang van hemosporidische infecties worden onderschat en verwaarloosd. We hebben een nieuwe multiplex PCR-assay ontwikkeld om gelijktijdigdetecteren en differentiëren tussen vier hemosporidische parasieten: Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare en Plasmodium gallinaceum. Primers in de huidige studie versterkten specifiek de overeenkomstige doelen zonder dat er tussen soorten werd amplificatie gedetecteerd.

De multiplex PCR vertoonde een important hogere detectiesnelheid in vergelijking met microscopisch onderzoek (p = 0,0001) . De resultaten tonen aan dat de detectiesnelheid van multiplex PCR voor L. sabrazesi, P. juxtanucleare en P. gallinaceum allemaal groter zijn dan die van microscopisch onderzoek met respectievelijk p = 0,002, 0,0001 en 0,004. Co-infecties werden ook effectiever gedetecteerd door multiplex PCR. We hebben de huidige methode toegepast op veldmonsters afkomstig uit de provincies Nan, Prachinburi en Chachoengsao. De huidige studie onthulde dat positieve percentages van hemosporidische parasieten bij kippen in de drie onderzoekslocaties varieerden van 39,5%-93,8%. De huidige take a look at biedt een tijdbesparende optie voor moleculaire diagnose in plaats van het gebruik van singleplex PCR’s voor detectiede parasieten afzonderlijk. Binnen een enkele reactie zou deze take a look at een nuttig hulpmiddel zijn voor de detectie van hemosporidische parasieten bij vogels, hetzij afzonderlijk, hetzij onder omstandigheden van gelijktijdige infectie, en voor grootschalige epidemiologische research.

 

De associatie van leeftijd, geslacht en RT-PCR-resultaten met het aantal cellen en neutrofielen in SARS-CoV-2-infectie: een dwarsdoor lymfesnede-analyse van 1450 Iraanse patiënten met COVID-19

Inperking van pandemische infecties hangt voornamelijk af van de snelle identificatie van dragers, mogelijk door strikt toezicht en waarheidsgetrouwe diagnostische exams. Hoewel moleculaire identificatie van ernstig acuut respiratoir syndroom coronavirus 2 (SARS-CoV-2) de gouden standaardmethode is, zijn de lage gevoeligheid en lange doorlooptijd een van de grootste zorgen. In deze retrospectieve studie in één centrum hebben we de resultaten beoordeeld van de lymfocyten- en neutrofielentellingen van 1450 Iraanse patiënten met coronavirusziekte 2019 (COVID-19), gerekruteerd in het Baqiyatallah-ziekenhuis, Teheran, Iran. Van de 1450 patiënten waren 439 gevallen (30,3% ) negatief voor de polymerasekettingreactie (PCR); verder benadrukkend dat het krijgen van negatieve moleculaire testen niet zo betrouwbaar is als een positief resultaat. Terwijl het aantal lymfocyten in gevallen met een leeftijd van minder dan 50 jaar 1,8 × 103/µL (1,2-2,5) was, was het 1,47 × 103/µL (0,84-2,16) in de oudere groep (p<0,001). Ook hadden mannen lagere lymfocyten in vergelijking met vrouwen (1,53×103/µL vs 1,76×103/µL; p=0,002). Van bijzonder belang was het aantal lymfocyten in de PCR-negatieve gevallen 1,77 × 103/µL (0,98-2,45), wat important hoger was dan het aantal in hun positieve tegenhangers (1,53 × 103/µL; p=0,004).

In tegenstelling tot lymfocyten hadden geslacht en PCR geen significante invloed op het aantal neutrofielen. De odds ratio voor neutrofilie bij patiënten ouder dan 50 jaar, met een negatieve of een positieve PCR, was 2,46 en 2,23, wat suggereert dat ouderdom de belangrijkste geassocieerde issue is. Het aantal lymfocyten samen met een verhoogd aantal neutrofielen kan waarschijnlijk dienen als eenvoudige, snelle en economische biomarkers, en het zijn schijnbaar geschikte objects waarmee rekening moet worden gehouden bij de identificatie van patiënten met COVID-19, vooral degenen die ouder zijn dan 50.

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)
RK21205	Abclonal	1 ml	Ask for price

2X Universal SYBR Green Fast qPCR Mix
RK21203	Abclonal	1 mL	Ask for price

Genious 2X SYBR Green Fast qPCR Mix
RK21204	Abclonal	1 mL	Ask for price

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
17270	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
17271	AAT Bioquest	5 mL	EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
17272	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
17273	AAT Bioquest	5 mL	EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
17274	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
17275	AAT Bioquest	5 mL	EUR 308

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17276	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
17277	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17278	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
17279	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17280	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
17281	AAT Bioquest	5 mL	EUR 308

Entrans 2X qPCR Probe Master Mix
RK21208	Abclonal	40 RXN	Ask for price

miRNA Universal SYBR® qPCR Master Mix
MQ101-01	Vazyme	125 rxn(20 μl/rxn)	EUR 165.6

miRNA Universal SYBR® qPCR Master Mix
MQ101-02	Vazyme	500 rxn(20 μl/rxn)	EUR 290.4

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001	GenDepot	1ml	EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-002	GenDepot	2x1ml	EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-005	GenDepot	5x1ml	EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-010	GenDepot	10x1ml	EUR 1632

HotStart PCR Master mix (2X, Green Dye)
BS9291	Bio Basic	5X1mL, 5ml	EUR 251.4

HotStart PCR Master mix (2X, Green Dye)
BS9292	Bio Basic	1ml	EUR 115.68

Bio-Star Mastermix with SYBR (2X) High-ROX
Y240	GeneOn	100 rcs (2,5 ml)	EUR 122.4

Bio-Star Mastermix with SYBR (2X) Low-ROX
Y250	GeneOn	100 rcs (2,5 ml)	EUR 122.4

Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX)
RK21206	Abclonal	1 ml	Ask for price

Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn)
31014	Biotium	2x1mL	EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
31014-1	Biotium	5x1mL	EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2	Biotium	50x1mL	EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)
31015-1	Biotium	5x1mL	EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2	Biotium	50x1mL	EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)
31042-1	Biotium	5x1mL	EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)
31042-20mL	Biotium	2x10mL	EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)
31042-T	Biotium	1mL	EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Taqman Master Mix-ROX
M1124-500	Biovision	each	EUR 529.2

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-001	GenDepot	1ml	EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-002	GenDepot	2x1ml	EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-005	GenDepot	5x1ml	EUR 322.8

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-010	GenDepot	10x1ml	EUR 537.6

amfiSure qGreen Q-PCR Master Mix(2X), No Rox
Q5600-050	GenDepot	50x1ml	EUR 2085.6

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-001	GenDepot	1ml	EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-002	GenDepot	2x1ml	EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-005	GenDepot	5x1ml	EUR 319.2

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-010	GenDepot	10x1ml	EUR 530.4

amfiSure qGreen Q-PCR Master Mix(2X), High Rox
Q5602-050	GenDepot	50x1ml	EUR 2004

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-001	GenDepot	1ml	EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-002	GenDepot	2x1ml	EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-005	GenDepot	5x1ml	EUR 319.2

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-010	GenDepot	10x1ml	EUR 530.4

amfiSure qGreen Q-PCR Master Mix(2X), Low Rox
Q5603-050	GenDepot	50x1ml	EUR 2004

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-001	GenDepot	1ml	EUR 208.8

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-002	GenDepot	2x1ml	EUR 319.2

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-005	GenDepot	5x1ml	EUR 562.8

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-010	GenDepot	10x1ml	EUR 886.8

amfiSure qProbe Q-PCR Master Mix(2X), No Rox
Q5700-050	GenDepot	50x1ml	EUR 3462

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-001	GenDepot	1ml	EUR 208.8

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-002	GenDepot	2x1ml	EUR 319.2

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-005	GenDepot	5x1ml	EUR 562.8

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-010	GenDepot	10x1ml	EUR 886.8

amfiSure qProbe Q-PCR Master Mix(2X), High Rox
Q5702-050	GenDepot	50x1ml	EUR 400.8

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-001	GenDepot	1ml	EUR 208.8

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-002	GenDepot	2x1ml	EUR 319.2

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-003	GenDepot	5x1ml	EUR 562.8

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-004	GenDepot	10x1ml	EUR 886.8

amfiSure qProbe Q-PCR Master Mix(2X), Low Rox
Q5703-005	GenDepot	50x1ml	EUR 3462

SYBR Green I
B1747-25	Biovision	each	EUR 1396.8

SYBR Green I
B1747-5	Biovision	each	EUR 464.4

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T	Biotium	1mL	EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
31015	Biotium	2x1mL	EUR 273.6
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL)
31015-T	Biotium	1mL	EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

Fast Probe Master Mix with Rox (200 rxn)
31016	Biotium	2x1mL	EUR 280.8
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)
31016-1	Biotium	5x1mL	EUR 559.2
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Probe Master Mix with Rox (5000 rxn)
31016-2	Biotium	50x1mL	EUR 4645.2
Description: Minimum order quantity: 1 unit of 50x1mL

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L	MiTeGen	960 reactions x 20 µl	EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S	MiTeGen	192 reactions x 20 µl	EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

Taqman Master Mix-Low ROX
M1123-500	Biovision	each	EUR 529.2

EZSolution? SYBR Green I
B1748-1	Biovision	each	EUR 744

EZSolution? SYBR Green I
B1748-500	Biovision	each	EUR 516

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17282	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
17283	AAT Bioquest	5 mL	EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
17284	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
17285	AAT Bioquest	5 mL	EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
17286	AAT Bioquest	1 mL	EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
17287	AAT Bioquest	5 mL	EUR 308

HotTaq Probe qPCR Mix (ROX)
BT11001	Bioatlas	250rxn	EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
BT11101	Bioatlas	250rxn	EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RT Master Mix for qPCR
HY-K0510	MedChemExpress	1 mL (100 rxns)	EUR 349.2

AceQ qPCR Probe Master Mix
Q112-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR Probe Master Mix
Q112-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

Pfu 2x Master Mix - 200 Reactions
3326	Intact Genomics	1/EA	EUR 360

amfiSure ONE PCR Master Mix(2X)
P7000-005	GenDepot	5x1 ml	EUR 262.8

amfiSure ONE PCR Master Mix(2X)
P7000-010	GenDepot	10x1 ml	EUR 380.4

amfiSure ONE PCR Master Mix(2X)
P7000-050	GenDepot	50x1 ml	EUR 1453.2

amfiSure ONE PCR Master Mix(2X)
P7000-100	GenDepot	100x1 ml	EUR 2522.4

Gloria Nova HS 2x Master Mix
RK20717	Abclonal	1mL	Ask for price

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045	Biotium	EUR 136.80 EUR 578.40 EUR 319.20	1mL 1X10mL 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)
9-31046	Biotium	EUR 136.80 EUR 578.40 EUR 319.20	1mL 1X10mL 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17288	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
17289	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17290	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
17291	AAT Bioquest	5 mL	EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17292	AAT Bioquest	1 mL	EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
17293	AAT Bioquest	5 mL	EUR 308

SensiFAST SYBR Lo-ROX Mix, 50 mL
BIO-94050	Bioline	5000 rxn	Ask for price

SensiFAST SYBR No-ROX Mix, 50 mL
BIO-98050	Bioline	5000 rxn	Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P	Yeastern Biotech	100 Preps 1.25 ml	Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P	Yeastern Biotech	400 Preps 1.25 ml x 4	Ask for price

Fast Probe Master Mix with Rox, trial size (100 rxn)
31016-T	Biotium	1mL	EUR 169.2
Description: Minimum order quantity: 1 unit of 1mL

HotTaq Probe qPCR Mix (no ROX)
BT11002	Bioatlas	250rxn	EUR 130.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)
BT11102	Bioatlas	250rxn	EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

SensiFAST Lyo-Ready No-ROX Mix, 2x
BIO-11060	Cusabio	10 x 10ml	Ask for price

SensiFAST Lyo-Ready No-ROX Mix, 2x
BIO-11061	Bioline	5ml	Ask for price

Genious 2X SYBRGREEN FASTqPCR MIX (High ROX)
RK21207	Abclonal	1 ml	Ask for price

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 227.04

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 227.04

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010	GenDepot	2x50 rxns	EUR 303.6

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-025	GenDepot	5X50 rxns	EUR 546

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-050	GenDepot	10X50 rxns	EUR 934.8

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-100	GenDepot	20x50 rxns	EUR 1599.6

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions)
31044-1	Biotium	1kit	EUR 396
Description: Minimum order quantity: 1 unit of 1kit

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions)
31044-T	Biotium	1kit	EUR 136.8
Description: Minimum order quantity: 1 unit of 1kit

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions	BosterBio	100 Reactions	EUR 392.4

Bio-Star Mastermix with SYBR (2X) BLUE
Y220	GeneOn	100 rcs (2,5 ml)	EUR 112.8

Accuris qMax Probe No Rox qPCR Mix
PR2001-N-1000	Benchmark Scientific	1 PC	EUR 561.29

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)
PR2120-N-25BLK	Benchmark Scientific	1 each	EUR 2695

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)
PR2120-N-50BLK	Benchmark Scientific	1 each	EUR 4785

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-001	GenDepot	1ml	EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-002	GenDepot	2x1ml	EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-005	GenDepot	5x1ml	EUR 319.2

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-010	GenDepot	10x1ml	EUR 530.4

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-050	GenDepot	50x1ml	EUR 2004

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-001	GenDepot	1ml	EUR 208.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-002	GenDepot	2x1ml	EUR 319.2

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-005	GenDepot	5x1ml	EUR 562.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-010	GenDepot	10x1ml	EUR 886.8

amfiSure qProbe Q-PCR Master Mix(2X), Fluorescein
Q5701-050	GenDepot	50x1ml	EUR 3462

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500
HS-100-QPCR	CORNING	100/pk	EUR 381.6
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500
HS-150-QPCR	CORNING	100/pk	EUR 381.6
Description: Sealing Products; Sealing films - Axygen

Accuris qMAX SYBR Green, No ROX, 2500 reactions (1x25ml)
PR2000-N-25BLK	Benchmark Scientific	1 each	EUR 1133

Accuris qMAX SYBR Green, No ROX, 5000 reactions (1x50ml)
PR2000-N-50BLK	Benchmark Scientific	1 each	EUR 2039.4

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210	GeneOn	100 rcs (2,5 ml)	EUR 165.6

qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials
S210L	GeneOn	500 rcs (12,5 ml)	EUR 627.6

Bio-Star Mastermix with SYBR (2X) no dye
Y210	GeneOn	100 rcs (2,5 ml)	EUR 106.8

SYBR Green I Nucleic Acid Gel Stain
HY-K1004	MedChemExpress	100 μL	EUR 135.6

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003	Biotium	2x1ML	EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)
31003-1	Biotium	5x1ML	EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Fast EvaGreen Master Mix for qPCR (5000 rxn)
31003-2	Biotium	50x1ML	EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast Plus EvaGreen qPCR Master Mix (500 rxn)
31020-1	Biotium	5x1mL	EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2	Biotium	50x1mL	EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Genorise® 2 x qPCR HotStart Master Mix
GR108002	Genorise Scientific	2.0 ml	EUR 334.8

RT Master Mix for qPCR (gDNA digester plus)
HY-K0511	MedChemExpress	1 mL (100 rxns)	EUR 378

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R	Bio Basic	4X1.25ml	EUR 303.25

ABScript II One Step SYBR Green RT-qPCR Kit
RK20404	Abclonal	10 RXN	Ask for price

Deoxy+ SYBR Green OneStep qRT-PCRKit wi ROX, 100 preps
FYT504-100P	Yeastern Biotech	1 vial	Ask for price

Deoxy+ SYBR Green OneStep qRT-PCRwi ROX Kit, 50 preps
FYT504-50P	Yeastern Biotech	1 vial	Ask for price

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170	GeneOn	100 rcs (2,5 ml)	EUR 165.6

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
S170L	GeneOn	500 rcs (12,5 ml)	EUR 627.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175	GeneOn	100 rcs (2,5 ml)	EUR 165.6

qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S175L	GeneOn	500 rcs (12,5 ml)	EUR 627.6

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215	GeneOn	100 rcs (2,5 ml)	EUR 190.8

qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials
S215L	GeneOn	500 rcs (12,5 ml)	EUR 627.6

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220	GeneOn	100 rcs (2,5 ml)	EUR 190.8

qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S220L	GeneOn	500 rcs (12,5 ml)	EUR 734.4

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225	GeneOn	100 rcs (2,5 ml)	EUR 190.8

qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials
S225L	GeneOn	500 rcs (12,5 ml)	EUR 734.4

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372L	MiTeGen	10 x 1,25 ml	EUR 720.29
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service
M-PCR-372S	MiTeGen	2 x 1,25 ml	EUR 180.14
Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service

ACCUZYME Mix, 2x
BIO-25028	Bioline	500 Reactions	Ask for price

MyTaq Mix, 2x
BIO-25041	Bioline	200 Reactions	Ask for price

MyTaq Mix, 2x
BIO-25041/S	Bioline	Sample	Ask for price

MyTaq Mix, 2x
BIO-25042	Bioline	1000 Reactions	Ask for price

Analyse en voorspelling van eenvoudige real-time CR-primers en probes voor SARS-CoV-2

Snelle exams voor actieve SARS-CoV-2-infecties zijn gebaseerd op reverse transcription polymerase chain response (RT-PCR). RT-PCR maakt gebruik van reverse transcriptie van RNA in complementair DNA (cDNA) en amplificatie van specifieke DNA- doelen (primer en probe) met behulp van polymerasekettingreactie (PCR). De technologie maakt snelle en specifieke identificatie van het virus mogelijk op foundation van sequentiehomologie van nucleïnezuursequentie en is veel sneller dan weefselkweek- of diercelmodellen. De techniek kan echter in de loop van de tijd aan gevoeligheid verliezen naarmate het virus evolueert en de doelsequenties afwijken van de selectieve primersequenties. Verschillende primersequenties zijn aangenomen in verschillende geografische regio’s.

Omdat we vertrouwen op deze bestaande RT-PCR-primers om de verspreiding van het Coronavirus te volgen en te beheersen, is het absoluut noodzakelijk om te begrijpen hoe SARS-CoV-2-mutaties, in de loop van de tijd en geografisch, afwijken van de bestaande primers die tegenwoordig worden gebruikt. In deze studie analyseren we de prestaties van de SARS-CoV-2-primers die tegenwoordig in gebruik zijn door het aantal mismatches tussen primersequentie en genoomdoelen in de tijd en ruimtelijk te meten. We stellen huge dat er een groeiend aantal mismatches is, een toename met 2% per maand , evenals een hoge specificiteit van het virus op foundation van geografische locatie

SARS-CoV-2 E-genvariant veranderde analytische gevoeligheidskenmerken van virale detectie met behulp van een commerciële RT-PCR-test

Diagnostische exams voor het detecteren van SARS-CoV-2 zijn essentieel voor patiëntenbeheer, infectiepreventie en de reactie van de volksgezondheid op COVID-19 . De werkzaamheid en betrouwbaarheid van deze exams zijn van het grootste belang bij het volgen en beheersen van de verspreiding van het virus. Realtime RT-PCR-assays zijn afhankelijk van een vaste genetische sequentie voor primers en probebinding. Mutaties kunnen mogelijk de nauwkeurigheid van deze testen veranderen en leiden tot onvoorspelbare analytische prestatiekenmerken en fout-negatieve resultaten. Hierin identificeren we een G-naar-U transversie (nucleotide 26372)in het SARS-CoV-2 E-gen in drie exemplaren met verminderde virale detectie-efficiëntie met behulp van een algemeen beschikbare commerciële take a look at. Verdere analyse van de openbare GISAID-repository leidde tot de identificatie van 18 further genomen met deze mutatie, die vijf onafhankelijke mutatiegebeurtenissen weerspiegelen. Dit werk ondersteunt het gebruik van dual-target assays om het aantal fout-negatieve PCR-resultaten te verminderen.

 

Argonaute nauwgezet PCR-systeem met één buis maakt overgevoelige detectie van grote mutaties mogelijk

Technologische vooruitgang in de detectie van zeldzame DNA-mutaties heeft een revolutie teweeggebracht in de diagnose en monitoring van tumoren, maar ze worden nog steeds beperkt door het gebrek aan supergevoelige en goed dekkende procedures voor het identificeren van mutaties met een lage overvloed. Hier beschrijven we een multiplex PCR-gebaseerd systeem met één buis, A-Star, waarbij een hyperthermofiel argonaute van Pyrococcus furiosus (PfAgo) betrokken is voor zeer efficiënte detectie van zeldzame mutaties die gunstig zijn vanwege de compatibiliteit met DNA-polymerase. Deze nieuwe techniek maakt gebruik van een specifieke strategie voor het ontwerpen van een gids om PfAgo-selectieve splitsing mogelijk te maken met een resolutie van één nucleotide bij 94 ° C, waardoor wild-type DNA grotendeels wordt geëlimineerd in de denaturatiestap en efficiënt zeldzaam gemuteerd DNA wordt geamplificeerd tijdens het PCR-proces.

Het geïntegreerde systeem met één buis bereikte een grote efficiëntie voor het verrijken van zeldzame mutaties in vergelijking met een verdeeld systeem dat de splitsing en amplificatie scheidt. A-Star maakt dus eenvoudige detectie en kwantificering van 0,01% zeldzame mutaties mogelijk met een ≥5500-voudige toename in efficiëntie. De haalbaarheid van A-Star werd ook aangetoond voor het detecteren van oncogene mutaties in solide tumorweefsels en bloedmonsters. Opmerkelijk genoeg bereikte A-Star gelijktijdige detectie van meerdere oncogenen by way of een eenvoudige reactie met één buis door orthogonale, op een gids gerichte specifieke splitsing. Deze studie demonstreert een supergevoelig en snel nucleïnezuurdetectiesysteem met veelbelovend potentieel voor zowel onderzoeks- als therapeutische toepassingen.

Relaxin 3 protein
30R-AR029	Fitzgerald	25 ug	EUR 327.6
Description: Purified recombinant Human Relaxin 3 protein

Anti-Relaxin 1 Antibody
STJ502816	St John's Laboratory	100 µg	EUR 571.2

Anti-Relaxin 1 (1H6)
YF-MA15197	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to Relaxin 1

Recombinant Human Relaxin-3 Protein
PROTQ8WXF3-1	BosterBio	25ug	EUR 380.4
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Relaxin H2 (human)
H-6784.0200	Bachem	0.2mg	EUR 741.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)
H-6784.1000	Bachem	1.0mg	EUR 2853.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)
H-7322.0200	Bachem	0.2mg	EUR 741.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)
H-7322.1000	Bachem	1.0mg	EUR 2853.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin 3 Polyclonal Antibody
ABP53672-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ABP53672-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ABP53672-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ES4671-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Relaxin 3 Polyclonal Antibody
ES4671-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Relaxin-3 Recombinant Protein
40-230-0005mg	ProSci	0.005 mg	EUR 311.1
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin-3 Recombinant Protein
40-230-0025mg	ProSci	0.025 mg	EUR 437.1
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as "somatostatin-like” or "angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an "orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Relaxin 3 (RLN3) Antibody
20-abx174368	Abbexa	EUR 427.20 EUR 1095.60 EUR 560.40 EUR 184.80 EUR 326.40	100 ug 1 mg 200 ug 20 ug 50 ug

Relaxin 3 Blocking Peptide
20-abx161415	Abbexa	EUR 727.20 EUR 1713.60	1 mg 5 mg

Relaxin 3 (RLN3) Antibody
20-abx121705	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

Relaxin 3 (RLN3) Antibody
20-abx130221	Abbexa	EUR 543.60 EUR 159.60 EUR 1562.40 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Relaxin 3 (RLN3) Antibody
20-abx323035	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Relaxin 3 (RLN3) Antibody
20-abx178243	Abbexa	EUR 1479.60 EUR 710.40	1 mg 200 ug

Relaxin 3 (RLN3) Antibody
20-abx178244	Abbexa	EUR 1479.60 EUR 710.40	1 mg 200 ug

Relaxin 3 (RLN3) Antibody
20-abx218313	Abbexa	EUR 510.00 EUR 410.40	100 ug 50 ug

Recombinant Relaxin 3 (RLN3)
4-RPD869Ra01	Cloud-Clone	EUR 588.79 EUR 280.80 EUR 1877.95 EUR 705.98 EUR 1291.97 EUR 469.20 EUR 4514.88	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Relaxin 3 expressed in: E.coli

Anti-Relaxin 1/RLN1 Antibody
A08367-1	BosterBio	100ug/vial	EUR 400.8

Anti-Relaxin Receptor 2 Antibody
A04848	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Relaxin Receptor 2 Antibody (RXFP2) detection.tested for WB in Human, Mouse, Rat.

Anti-Relaxin 1 Antibody BIOTIN
STJ502817	St John's Laboratory	100 µg	EUR 703.2

Anti-Relaxin 1 Antibody FITC
STJ502818	St John's Laboratory	100 µg	EUR 703.2

Anti-Relaxin Receptor 2 antibody
STJ95407	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to Relaxin Receptor 2.

Anti-Relaxin Receptor 4 antibody
STJ95409	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to Relaxin Receptor 4.

Human Relaxin 3 PicoKine ELISA Kit
EK1407	BosterBio	96 wells	EUR 510
Description: For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin).

ELISA kit for Human Relaxin 3
EK5653	SAB	96 tests	EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Relaxin 3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Relaxin 3 (RLN3) ELISA Kit
abx512123-96tests	Abbexa	96 tests	EUR 904.8

Human Relaxin 3 (RLN3) ELISA Kit
abx253713-96tests	Abbexa	96 tests	EUR 904.8

Human RLN3(Relaxin-3) ELISA Kit
EH0755	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human RLN3/ Relaxin-3 ELISA Kit
E2158Hu	Sunlong	1 Kit	EUR 685.2

RLN3 Relaxin-3 Human Recombinant Protein
PROTQ8WXF3	BosterBio	Regular: 25ug	EUR 380.4
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques.

Human Relaxin 3(RLN3)ELISA Kit
QY-E01376	Qayee Biotechnology	96T	EUR 433.2

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 336

Anti-VEGF Receptor 3/FLT4 Antibody
A01276-3	BosterBio	100ug/vial	EUR 400.8

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
M02431-3	BosterBio	100ug/vial	EUR 476.4
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-Human IBA1 Antibody
M01394-3	BosterBio	100ul	EUR 476.4
Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human.

Anti-Human Ki67 Antibody
M00254-3	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Human Ki67 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
M00621-3	BosterBio	100ug/vial	EUR 400.8

Anti-active Caspase-3 Rabbit Monoclonal Antibody
M00334-3	BosterBio	100ug/vial	EUR 476.4
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Human Relaxin ELISA kit
E01R0008-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin (RLN) Protein
20-abx654931	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Relaxin ELISA Kit
ELA-E1216h	Lifescience Market	96 Tests	EUR 988.8

Relaxin-2, human recombinant
7194-10	Biovision	each	EUR 248.4

Relaxin-2, human recombinant
7194-50	Biovision	each	EUR 810

Relaxin Receptor 3 Polyclonal Antibody
ES6513-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody
ES6513-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin 3 receptor 1 Antibody
48026-100ul	SAB	100ul	EUR 399.6

Relaxin 3 receptor 1 Antibody
48026-50ul	SAB	50ul	EUR 286.8

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Rat Relaxin 3 (RLN3) Protein
20-abx168490	Abbexa	EUR 828.00 EUR 343.20 EUR 2532.00 EUR 978.00 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Relaxin 3 receptor 1 Antibody
abx019161-100ug	Abbexa	100 ug	EUR 410.4

Mouse Relaxin 3 (RLN3) Protein
20-abx654935	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Relaxin 3 (RLN3) Protein
20-abx654936	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Relaxin-3,RLN-3 ELISA
YLA1678RA-48T	Shanghai YL Biotech	48T	EUR 465

Rat Relaxin-3,RLN-3 ELISA
YLA1678RA-96T	Shanghai YL Biotech	96T	EUR 600

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody
M00227-3	BosterBio	100ul	EUR 476.4
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

ELISA kit for Human RLN3 (Relaxin-3)
E-EL-H1652	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-48T	Abbkine	48T	EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-96T	Abbkine	96T	EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Nori® Human Relaxin-3 ELISA Kit
GR111288	Genorise Scientific	96-well	EUR 553.2

Porcine relaxin peptides / relaxin (RLN) ELISA Kit
QY-E40036	Qayee Biotechnology	96T	EUR 480

Anti-MRP1 (human) Monoclonal Antibody (QCRL-1)
M00872-3	BosterBio	1ml	EUR 810
Description: Mouse Monoclonal MRP1 (human) Antibody (QCRL-1). Validated in IP, IF and tested in Human.

Recombinant Human MMP-3 Protein
PROTP08254-3	BosterBio	10ug	EUR 380.4
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121
M07469-3	BosterBio	100ug	EUR 450
Description: Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121 tested in ICC, IHC, FC, ELISA, reactive to Human

IL-3 Interleukin-3 Human Recombinant Protein, His Tag
PROTP08700-3	BosterBio	Regular: 50ug	EUR 380.4
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Human Relaxin (RLN) ELISA Kit
CEB216Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5402.92
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Relaxin (RLN) in serum, plasma and other biological fluids.

Human Relaxin (RLN) ELISA Kit
CEB216Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 550.13
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Relaxin (RLN) in serum, plasma and other biological fluids.

Human Relaxin (RLN) ELISA Kit
CEB216Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 734.46
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Relaxin (RLN) in serum, plasma and other biological fluids.

Human Relaxin (RLN) ELISA Kit
CEB216Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2945.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Relaxin (RLN) in serum, plasma and other biological fluids.

Human Relaxin (RLN) ELISA Kit
4-CEB216Hu	Cloud-Clone	EUR 5463.60 EUR 2886.00 EUR 735.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Relaxin (RLN) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Relaxin, RLN ELISA Kit
CSB-E09157h-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Relaxin, RLN in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Relaxin, RLN ELISA Kit
1-CSB-E09157h	Cusabio	EUR 1080.00 EUR 6571.20 EUR 3480.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Relaxin, RLN in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Relaxin,RLN ELISA Kit
CN-04606H1	ChemNorm	96T	EUR 529.2

Human Relaxin,RLN ELISA Kit
CN-04606H2	ChemNorm	48T	EUR 349.2

Human Relaxin (RLN) ELISA Kit
DLR-RLN-Hu-48T	DL Develop	48T	EUR 597.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Relaxin (RLN) in samples from serum, plasma or other biological fluids.

Human Relaxin (RLN) ELISA Kit
DLR-RLN-Hu-96T	DL Develop	96T	EUR 776.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Relaxin (RLN) in samples from serum, plasma or other biological fluids.

Human Relaxin 2 (RLN2) Protein
20-abx068856	Abbexa	EUR 828.00 EUR 343.20 EUR 2548.80 EUR 978.00 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Relaxin (RLN) ELISA Kit
20-abx152946	Abbexa	EUR 8534.40 EUR 4550.40 EUR 1054.80	10 × 96 tests 5 × 96 tests 96 tests

Human Relaxin (RLN) ELISA Kit
20-abx051409	Abbexa	EUR 8534.40 EUR 4550.40 EUR 1054.80	10 × 96 tests 5 × 96 tests 96 tests

Human Relaxin (RLN) CLIA Kit
20-abx490381	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Human Relaxin,RLN ELISA Kit
201-12-1325	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Relaxin (RLN) Monoclonal Antibody (Human)
4-MAB216Hu22	Cloud-Clone	EUR 296.40 EUR 3027.60 EUR 753.60 EUR 373.20 EUR 256.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Relaxin (RLN)

Relaxin-2 (Human) ELISA Kit
E4824-100	Biovision	96 assays	EUR 903.6

Human Relaxin(RLN)ELISA Kit
GA-E1341HM-48T	GenAsia Biotech	48T	EUR 346.8

Human Relaxin(RLN)ELISA Kit
GA-E1341HM-96T	GenAsia Biotech	96T	EUR 559.2

Recombinant Human Relaxin-2 Protein
PROTP04090-2	BosterBio	25ug	EUR 380.4
Description: Relaxin-2 is a peptide hormone structurally related to insulin, which is expressed in the placenta, decidua, prostate, and in the ovary during pregnancy. Of the three known relaxin genes, Relaxin-2 is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 and LGR8 receptors, previously identified as an “orphan” G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Recombinant Relaxin-2 is a nonglycosylated 6.0 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.

Human Relaxin (RLN) ELISA Kit
RD-RLN-Hu-48Tests	Reddot Biotech	48 Tests	EUR 600

Human Relaxin (RLN) ELISA Kit
RD-RLN-Hu-96Tests	Reddot Biotech	96 Tests	EUR 830.4

Human Relaxin(RLN)ELISA Kit
QY-E01378	Qayee Biotechnology	96T	EUR 433.2

Human Relaxin (RLN) ELISA Kit
RDR-RLN-Hu-48Tests	Reddot Biotech	48 Tests	EUR 626.4

Human Relaxin (RLN) ELISA Kit
RDR-RLN-Hu-96Tests	Reddot Biotech	96 Tests	EUR 868.8

Human Relaxin,RLN ELISA Kit
YLA1773HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Relaxin,RLN ELISA Kit
YLA1773HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Relaxin 1 antibody
70R-12153	Fitzgerald	100 ug	EUR 501.6
Description: Rabbit polyclonal Relaxin 1 antibody

Relaxin 2 antibody
70R-12180	Fitzgerald	200 ug	EUR 483.6
Description: Rabbit polyclonal Relaxin 2 antibody

Relaxin 2 protein
30R-AR028	Fitzgerald	25 ug	EUR 327.6
Description: Purified recombinant Human Relaxin 2 protein

Relaxin (RLN) Antibody
20-abx100116	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Relaxin (RLN) Antibody
20-abx174364	Abbexa	EUR 1045.20 EUR 543.60	1 mg 200 ug

Relaxin (RLN) Antibody
20-abx174365	Abbexa	EUR 1045.20 EUR 543.60	1 mg 200 ug

Relaxin (RLN) Antibody
20-abx174366	Abbexa	EUR 1128.00 EUR 577.20	1 mg 200 ug

Relaxin (RLN) Antibody
20-abx174367	Abbexa	EUR 1128.00 EUR 577.20	1 mg 200 ug

Relaxin (RLN) Antibody
20-abx132050	Abbexa	EUR 393.60 EUR 978.00 EUR 510.00 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Relaxin (RLN) Antibody
20-abx130339	Abbexa	EUR 526.80 EUR 159.60 EUR 1496.40 EUR 710.40 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Relaxin 1 Antibody
abx021281-10ug	Abbexa	10 ug	EUR 510

Relaxin (RLN) Antibody
20-abx178238	Abbexa	EUR 1378.80 EUR 678.00	1 mg 200 ug

Relaxin (RLN) Antibody
20-abx178239	Abbexa	EUR 1412.40 EUR 693.60	1 mg 200 ug